Part:BBa_J45006

alcohol acetyltransferase I (ATF1)

Alcohol acetyltransferase I (ATFI) encodes the enzyme alcohol acetyltransferase I (AATase I), which produces acetate esters from acetyl CoA and alcohols. It was obtained from S. cerevisiae.

Usage and Biology

We will be interested in using AATase I to catalyze the reaction: isoamyl alcohol -> isoamyl acetate. Isoamyl acetate is a volatile ester with a strong banana-like scent. (The related enzyme AATase II, which is encoded by the gene ATF2, performs a similar function...but it is less efficient.)

According to studies done by [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12937998&query_hl=16&itool=pubmed_docsum Horton et al.], the addition of 10mM isoamyl alcohol to cultured E. coli expressing the ATF1 gene yields 1.8mM isoamyl acetate after 40 hours.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 99
  Illegal BamHI site found at 1426
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Unknown
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 526
  Illegal SapI.rc site found at 1534