Coding

Part:BBa_K3408001

Designed by: Fengzhi Yuan   Group: iGEM20_NAU-CHINA   (2020-10-03)
Revision as of 04:56, 22 October 2020 by NJAU-Chappie (Talk | contribs)

Phy(ycD)(Phytase)

It is the enzyme which could fix lead efficiently in a middle pH environment.

Because of the limitation of pH, we have to choose the phosphate-solubilizing enzyme which could work efficiently between the pH from pH6.0 to pH7.0. And we have found the Phy(ycD) whose optimum reaction pH is pH6.5. Thus, we added it into the anaerobic regulation circuit, following the Pnar.

T--NAU-CHINA--RA.jpg

Fig.2. Phy (ycD) activity changes with temperature(Wang Xi, et al., Applied and Environmental Biology. 2014)


According to the paper, we could see its optimum reaction temperature is 45℃, Km is 0.213mmol/L and Vmax is 5.55U/mg.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 359
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None