Part:BBa_K2717007

RBS2000-gdh-v5 tag-his tag

This part is a composite one, in which Promoter lacI and RBS 2000 control the glucose dehydrogenase and V5 tag and His tag. Rbs2000 can strengthen the expression of glucose dehydrogenase. Tag can be used to purify the proteins and to detect the expression of proteins. We constructed this vector to verify the growth-promoting effect of glucose dehydrogenase and its ability to retain plasmids.

function:

We designed this part can make gdh overexpressed due to RBS2000, and purify the glucose dehydrogenase protein with the help of the V5 tag and His tag and detect its expression. After confirming the expression of gdh, we used the same strain, and under the induction of IPTG, quantitatively detected the growth of the cells and the retention of the plasmid to verify the growth-promoting effect and plasmid retention of glucose dehydrogenase.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1569
  Illegal AgeI site found at 2461
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2443