![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3506030
Designed by: Yiying Yu Group: iGEM20_BNU-China (2020-10-21)
The cassette of periodic expression and degradation of Cas9
This part consists of four parts: CLB2 promoter, the first 124 amino acids of Clb2, a linker and Cas9. CLB2 promoter is a cell-cycle associated promoter and make Cas9 expressed in the S phase and reached its peak in the G2/M phase. The first 124 amino acids of Clb2 contains D-box and KEN-box, which are the recognition site by E3 ubiquitin ligase APC. The APC mediate the ubiquitin-proteasome proteolysis, making Cas9 degraded quickly. By this way, we control the expression of Cas9 coupled to cell division.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 229
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1578
Illegal BglII site found at 2373
Illegal BamHI site found at 2667
Illegal XhoI site found at 203
Illegal XhoI site found at 3173 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2405
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1496
Illegal BsaI.rc site found at 2738
Illegal BsaI.rc site found at 4094
Illegal BsaI.rc site found at 4517
Illegal BsaI.rc site found at 4529
Illegal BsaI.rc site found at 5396
Illegal SapI.rc site found at 4201
Illegal SapI.rc site found at 4783
Illegal SapI.rc site found at 4798
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Categories
Parameters
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