Composite

Part:BBa_K3506030

Designed by: Yiying Yu   Group: iGEM20_BNU-China   (2020-10-21)
Revision as of 14:57, 21 October 2020 by Registry (Talk | contribs)

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The cassette of periodic expression and degradation of Cas9

This part consists of four parts: CLB2 promoter, the first 124 amino acids of Clb2, a linker and Cas9. CLB2 promoter is a cell-cycle associated promoter and make Cas9 expressed in the S phase and reached its peak in the G2/M phase. The first 124 amino acids of Clb2 contains D-box and KEN-box, which are the recognition site by E3 ubiquitin ligase APC. The APC mediate the ubiquitin-proteasome proteolysis, making Cas9 degraded quickly. By this way, we control the expression of Cas9 coupled to cell division.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 229
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1578
    Illegal BglII site found at 2373
    Illegal BamHI site found at 2667
    Illegal XhoI site found at 203
    Illegal XhoI site found at 3173
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2405
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1496
    Illegal BsaI.rc site found at 2738
    Illegal BsaI.rc site found at 4094
    Illegal BsaI.rc site found at 4517
    Illegal BsaI.rc site found at 4529
    Illegal BsaI.rc site found at 5396
    Illegal SapI.rc site found at 4201
    Illegal SapI.rc site found at 4783
    Illegal SapI.rc site found at 4798


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