Coding
ClyR

Part:BBa_K3378000:Design

Designed by: Shengkun Tong   Group: iGEM20_HZAU-China   (2020-10-03)
Revision as of 14:49, 21 October 2020 by TSK (Talk | contribs) (→‎References)


Chimeric lysin ClyR with activity against Streptococcus mutans.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 757
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 121
    Illegal AgeI site found at 205
    Illegal AgeI site found at 520
    Illegal AgeI site found at 703
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of this part has been optimized based on E. coli codon bias.

Source

The catalytic domain come from Streptococcus virus C1 and the cell-wall binding domain come from Streptococcus suis.

References

[1] Yang, Hang, et al. "A chimeolysin with extended-spectrum streptococcal host range found by an induced lysis-based rapid screening method." Scientific reports 5 (2015): 17257.

[2] Yang, Hang, et al. "Antibiofilm activities of a novel chimeolysin against Streptococcus mutans under physiological and cariogenic conditions." Antimicrobial agents and chemotherapy 60.12 (2016): 7436-7443.

[3] Xu, Jingjing, et al. "Activity of the chimeric lysin ClyR against common Gram-positive oral microbes and its anticaries efficacy in rat models." Viruses 10.7 (2018): 380.