Coding

Part:BBa_K925005

Designed by: Michael Paul Lockhart   Group: iGEM12_St_Andrews   (2012-09-24)
Revision as of 14:49, 21 October 2020 by PC316 (Talk | contribs)

PdTagGST

This part produces functioning glutathione S-transferase (GST) protein that has a short petide sequence that binds palladium attached to the end of it. This allows the part to function as a palladium scavenger as the GST end can be bound to GST beads, while the palladium binding end is free to bind to any free Pd in solution.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 292
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 292
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 292
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 292
  • 1000
    COMPATIBLE WITH RFC[1000]


Description

This part was designed to provide a practical means for palladium scavenging. The GST end of the protein would be bound to beads and the palladium end free and able to bind. This would allow aqueous solution to be passed down a collum of GST beads with any free palladium being bound to the double ended protein.

Characterisation

No characterisation was undertaken.

Results

No results have been found.

Conclusion

This part has been designed and ligated but no expression or characterisation work has been done.

Contribution

Group: HK_SSC, 2019
Author: LEE Hong Kiu, CHOI Justin Yuet Hei

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Categories
Parameters
None