Coding

Part:BBa_K3512001

Designed by: Gourav Saha   Group: iGEM20_BITSPilani-Goa_India   (2020-10-20)
Revision as of 12:07, 21 October 2020 by Gourav saha (Talk | contribs)


ccdB Toxin

The CcdB toxin is part of the CcdA-CcdB system. The target of CcdB is the GyrA subunit of DNA gyrase, an essential type II topoisomerase in Escherichia coli. Gyrase alters DNA topology by effecting a transient double-strand break in the DNA backbone, passing the double helix through the gate and resealing the gaps. The CcdB toxin acts by trapping DNA gyrase in a cleaved complex with the gyrase A subunit covalently closed to the cleaved DNA, causing DNA breakage and cell death in a way closely related to quinolones antibiotics.

In absence of the antitoxin CcdA, the CcdB toxin traps DNA-gyrase cleavable complexes, inducing breaks into DNA and cell death.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 216


[edit]
Categories
//biosafety
//biosafety/kill_switch
//function/celldeath
Parameters
dissociation_constantHigh affinity = 3.57*10^-3 nM
proteinDissociation complex = 1.36*10^-9 nM