Generator

Part:BBa_K131019

Designed by: Thane Kubik   Group: iGEM08_Calgary_Wetware   (2008-10-27)
Revision as of 01:10, 29 October 2008 by DCurran (Talk | contribs)

LuxO inducible RFP

p_qrr4+Q04510+I3502

Usage and Biology

This is our empty response circuit for the quorum signaling molecule, AI-2. This was to go into our champion cell, along with the sensing circuit for AI-2, which contains, under regulation, the protein necessary to activate this circuit, LuxO (BBa_K131016). When LuxO is dephosphorylated, which occurs when AI-2 is present, transcription stops from BBa_K131017, leading to no more cl lam, and hence no more repression on the cl lam promoter. Thus, RFP is produced only when AI-2 is sensed. This construct has no terminators, as our champion cell is intended to produce both RFP and colicin E3 (an antibiotic).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1851
    Illegal AgeI site found at 1963
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None