Regulatory

Part:BBa_K3506011:Design

Designed by: Jiangyue Zhang   Group: iGEM20_BNU-China   (2020-10-21)
Revision as of 11:43, 21 October 2020 by Zhangjiangyue (Talk | contribs) (→‎References)


GAL1 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 70
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It's directly on pYES2 Vector. When using galactose to induce expression, make sure that galactose is the only carbon source in the medium.



Source

From pYES2 Vector.

References

[1] Johnston M. (1987). A model fungal gene regulatory mechanism: the GAL genes of Saccharomyces cerevisiae. Microbiological reviews, 51(4), 458–476. [2]Weinhandl, K., Winkler, M., Glieder, A., & Camattari, A. (2014). Carbon source dependent promoters in yeasts. Microbial cell factories, 13, 5. https://doi.org/10.1186/1475-2859-13-5