Part:BBa_K3506010
Clb2 N124aa
Over the four mitotic cyclins, cyclin b2 has the most important role in the yeast Saccharomyces cerevisiae, which is sufficient to trigger all essential functions of cyclin-dependent kinases in mitosis by itself. Research shows that a Clb2 KEN box can only degrade targeted fusion protein with the help of the first 124 amino acids of Clb2. So during MA and G1, a sequence of Clb2 124aa containing the KEN box and the destruction box act together to regulate proteolysis of Clb2 fusion protein.[1]
Biology and Usage
Clb2 N124aa is a sequence of the first 124 amino acids of Cyclin B2, derive from the geome of Saccharomyces cerevisiae BY4741, of which the function is to help the degradation of Clb2 fusion proten effectively during MA and G1 phase. This part can be used when you need to degrade your infusion protein with cyclin b2 coupled to cell circle, especially between MA and G1 phase.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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