Coding

Part:BBa_K3381002:Design

Designed by: Jeriann Hsiao   Group: iGEM20_Waterloo   (2020-10-20)
Revision as of 01:26, 21 October 2020 by Jlhsiao (Talk | contribs)


Mst-CopC (copper-binding)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The periplasmic localization tags were determined by the SignalP-5.0 Server and removed. The sequences were then codon optimized for E. coli, removing all cut sites for RFc 25 and NdeI/BamHI (required for cloning into pET 11a). Lastly, the prefix and suffix for RFc 25 were appended.


Source

Methylosinus trichosporium O3B, sequence found through GenBank https://www.ncbi.nlm.nih.gov/protein/ATQ66671

References