Coding

Part:BBa_K3381001:Design

Designed by: Alina Arvisais   Group: iGEM20_Waterloo   (2020-10-20)
Revision as of 01:24, 21 October 2020 by Jlhsiao (Talk | contribs) (→‎References)

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FutA2 (iron-binding)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Design Considerations: The periplasmic localization tags were determined by the SignalP-5.0 Server and removed. The sequences were then codon optimized for E. coli, removing all cut sites for RFc 25 and NdeI/BamHI (required for cloning into pET 11a). Lastly, the prefix and suffix for RFc 25 were appended.


Source

Source of this part: Synechocystis sp. PCC6803, sequence found through GenBank https://www.ncbi.nlm.nih.gov/nuccore/BA000022.2?from=2924779&to=2925819


References

Badarau, A., Firbank, S.J., Waldron, K.J., Yanagisawa, S., Robinson N.J., Banfield, M.J., & Dennison, C. (2008). FutA2 is a ferric binding protein from Synechocystis PCC 6803. Journal of Biological Chemistry, 283(18), 12520-7. https://doi.org/10.1074/jbc.M709907200

Katoh, H., Hagino, N., & Ogawa, T. (2001). Iron-Binding Activity of FutA1 Subunit of an ABC-type Iron Transporter in the Cyanobacterium Synechocystis sp. Strain PCC 6803. Plant and Cell Physiology, 42(8). 823–827, https://doi.org/10.1093/pcp/pce106

UniProtKB. (2020). Iron uptake protein A2. UniProt. https://www.uniprot.org/uniprot/Q55835