Coding

Part:BBa_K3381000:Design

Designed by: Jeriann Hsiao   Group: iGEM20_Waterloo   (2020-10-20)
Revision as of 01:12, 21 October 2020 by Jlhsiao (Talk | contribs) (→‎References)


FutA1 (iron-binding)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 414
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The periplasmic localization tags were determined by the SignalP-5.0 Server and removed. The sequences were then codon optimized for *E. coli*, removing all cut sites for RFc 25 and NdeI/BamHI (required for cloning into pET 11a). Lastly, the prefix and suffix for RFc 25 were appended.


Source

Synechocystis sp. PCC6803, sequence found through GenBank: https://www.ncbi.nlm.nih.gov/nuccore/BA000022.2?from=291626&to=292708

References

Badarau, A., Firbank, S.J., Waldron, K.J., Yanagisawa, S., Robinson N.J., Banfield, M.J., & Dennison, C. (2008). FutA2 is a ferric binding protein from Synechocystis PCC 6803. *Journal of Biological Chemistry, 283*(18), 12520-7. https://doi.org/10.1074/jbc.M709907200

Katoh, H., Hagino, N., & Ogawa, T. (2001). Iron-Binding Activity of FutA1 Subunit of an ABC-type Iron Transporter in the Cyanobacterium Synechocystis sp. Strain PCC 6803. *Plant and Cell Physiology, 42*(8). 823–827, https://doi.org/10.1093/pcp/pce106

Koropatkin, N. et al. (2007). The Structure of the Iron-binding Protein, FutA1, from Synechocystis 6803. *Journal of Biological Chemistry, 282*, 27468-27477. https://10.1074/jbc.M704136200