![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K3381000:Design
Designed by: Jeriann Hsiao Group: iGEM20_Waterloo (2020-10-20)
FutA1 (iron-binding)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 414
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The periplasmic localization tags were determined by the SignalP-5.0 Server and removed. The sequences were then codon optimized for *E. coli*, removing all cut sites for RFc 25 and NdeI/BamHI (required for cloning into pET 11a). Lastly, the prefix and suffix for RFc 25 were appended.
Source
Synechocystis sp. PCC6803, sequence found through GenBank: https://www.ncbi.nlm.nih.gov/nuccore/BA000022.2?from=291626&to=292708