Device

Part:BBa_K142047:Design

Designed by: Julius Rabl   Group: iGEM08_ETH_Zurich   (2008-10-28)
Revision as of 23:48, 28 October 2008 by Jrabl (Talk | contribs) (→‎Source)

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tet-controlled LacI generator with constitutive TetR expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1385
    Illegal NheI site found at 1408
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We decided to put the constitutive expression cassette behind the inducible LacI generator to minimze readthrough from the constitutive expression.


Source

Assembled from parts K142046 and I739001 by standard cloning techniques. Please see [http://2008.igem.org/Team:ETH_Zurich/Wetlab/Switch_Circuit ETH Zurich Wiki] for detailed documentation.

References