Part:BBa_K2226003

COX-2 gene

This part consists of the gene for cyclooxygenase-2 (COX-2), an enzyme that is found in elevated levels during inflammation, predominantly found in gastric mucosa and kidneys. Used to construct a plasmid that would produce COX-2 in the cell to simulate conditions in the body of patients with colorectal cancer.

References

Kloeckener-Gruissem, B., McEwen, J. E., & Poyton, R. O. (1987). Nuclear functions required for cytochrome c oxidase biogenesis in Saccharomyces cerevisiae: multiple trans-acting nuclear genes exert specific effects on expression of each of the cytochrome c oxidase subunits encoded on mitochondrial DNA. Current genetics, 12(5), 311–322. https://doi.org/10.1007/BF00405753

Taanman, J. W., & Capaldi, R. A. (1992). Purification of yeast cytochrome c oxidase with a subunit composition resembling the mammalian enzyme. The Journal of biological chemistry, 267(31), 22481–22485.

Geier, B. M., Schägger, H., Ortwein, C., Link, T. A., Hagen, W. R., Brandt, U., & Von Jagow, G. (1995). Kinetic properties and ligand binding of the eleven-subunit cytochrome-c oxidase from Saccharomyces cerevisiae isolated with a novel large-scale purification method. European journal of biochemistry, 227(1-2), 296–302. https://doi.org/10.1111/j.1432-1033.1995.tb20388.x

Cooper, C. E., Nicholls, P., & Freedman, J. A. (1991). Cytochrome c oxidase: structure, function, and membrane topology of the polypeptide subunits. Biochemistry and cell biology = Biochimie et biologie cellulaire, 69(9), 586–607. https://doi.org/10.1139/o91-089 Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]