Coding

Part:BBa_K136046:Design

Designed by: alexandra bouaziz   Group: iGEM08_Paris   (2008-10-25)
Revision as of 23:24, 28 October 2008 by Hs00590 (Talk | contribs) (→‎References)

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envZ*


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 316
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 316
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 316
    Illegal BglII site found at 236
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 316
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 316
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR and ligation in pCR4Blunt-TOPO


Source

bacterial genome of mutated (I281S) MG1655 strain for EnvZ.

  • Dissecting the Genetic Components of Adaptation of Escherichia coli to the Mouse Gut

Giraud A, Arous S, Gaboriau-Routhiau V, De Paepe M, Bambou JC, et al. PLoS Genetics January 2008

References

  • EnvZ-OmpR Interaction and Osmoregulation in Escherichia coli,

Sheng Jian Cai and Masayori Inouye (2002)

  • THE JOURNAL OF BIOLOGICAL CHEMISTRY, Vol. 277, No. 27