Part:BBa_K3582004:Design

The DBLb domain of PF11_0521 PfEMP1

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1407
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1113
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

• 1] RFC10 criteria satisfied
• 2] 6x-His-tag gene added for purification
• 3] Codon optimized with E-coli K-12 strain

Source

Protein sequence was obtained from PDB and reverse translated to give this biobrick

References

• [1] Baratin, M., Roetynck, S., Pouvelle, B., Lemmers, C., Viebig, N. K., Johansson, S., . . . Ugolini, S. (2007, February 21). Dissection of the role of PfEMP1 and ICAM-1 in the sensing of Plasmodium-falciparum-infected erythrocytes by natural killer cells. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1794133/
• [2]Hviid L, Jensen AT. PfEMP1 ‐ A parasite protein family of key importance in Plasmodium falciparum malaria immunity and pathogenesis. Adv Parasitol. 2015;88:51‐84
• [3]Staunton DE, Marlin SD, Stratowa C, Dustin ML, Springer TA. Primary structure of ICAM-1 demonstrates interaction between members of the immunoglobulin and integrin supergene families. Cell. 1988;52:925–933.
• [4]A. Bengtsson et a., A novel domain cassette identifies Plasmodium falciparum PfEMP1 proteins binding ICAM-1 and is a target of cross-reactive, adhesion-inhibitory antibodies. J. Immunol. 190, 240–249 (2013).
• [5]E. Carrington et al., In silico guided reconstruction and analysis of ICAM-1-binding var genes from Plasmodium falciparum. Sci. Rep. 8, 3282 (2018)
• [6] D. P. G. Howell et al., Mapping a common interaction site used by Plasmodium falciparum Duffy binding-like domains to bind diverse host receptors. Mol. Microbiol. 67, 78–87 (2008).CrossRefPubMedGoogle Scholar
• [7] H. Janes et al., Investigating the host binding signature on the Plasmodium falciparum PfEMP1 protein family. PLoS Pathog. 7, e1002032 (2011).