Protein_Domain
Part:BBa_K3582000:Design
Designed by: Saillesh Aravindhan C Group: iGEM20_IISER-Pune-India (2020-10-20)
ICAM-1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
- 1] Strep-tag was added at the end of the biobrick for ease of purification.
- 2] The sequence was codon optimized with the E.coli K-12 strain.
- 3] RFC10 criteria satisfied
Source
The protein sequence was taken from PDB and was reverse translated to give this biobrick
References
- [1] Abraham G, Colonno RJ (Aug 1984). "Many rhinovirus serotypes share the same cellular receptor". Journal of Virology. 51 (2): 340–45. doi:10.1128/JVI.51.2.340-345.1984. PMC 254443. PMID 6086949.
- [2] Rothlein R, Dustin ML, Marlin SD, Springer TA (August 1986). "A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1". Journal of Immunology. 137 (4): 1270–4. PMID 3525675.
- [3]Yang L, Froio RM, Sciuto TE, Dvorak AM, Alon R, Luscinskas FW (July 2005). "ICAM-1 regulates neutrophil adhesion and transcellular migration of TNF-alpha-activated vascular endothelium underflow". Blood. 106 (2): 584–92. doi:10.1182/blood-2004-12-4942. PMC 1635241. PMID 15811956
- [4] Staunton DE, Marlin SD, Stratowa C, Dustin ML, Springer TA. Primary structure of ICAM-1 demonstrates interaction between members of the immunoglobulin and integrin supergene families. Cell. 1988;52:925–933.
- [5]https://www.uniprot.org/uniprot/P05362