Coding
gal-3

Part:BBa_K1319003:Design

Designed by: Michael Osthege   Group: iGEM14_Aachen   (2014-09-25)
Revision as of 15:54, 20 October 2020 by Lavanya karinje (Talk | contribs) (→‎Source)

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human galectin-3, codon optimized for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 112
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part sequence was designed to fit with RFC25 prefix and suffix sequences, but contains a double stop codon at the native C-terminus because that's where the binding domain is located.


Source

This part was created by codon-optimization from the amino acid sequence of human galectin-3.

Organism: Homo sapiens (human)

References