Part:BBa_K3582103

Inhibitory Sequence 1 for PfEMP1-EPCR interaction

The following characteristic properties are observed in the inhibitory peptide sequence:

• 1] Interaction Energy:-15.7467 kcal/mol
• 2] Stability Value: 8.03145 dGunits

This bio brick synthesizes the inhibitory peptide sequence that is believed to bind more efficiently as Compared to the wild type sequence. The peptide sequence is built using saturated mutagenesis. In this particular mutant sequence, the amino acid of the Wild type sequence-Leucine(L) is replaced with Tryptophan(W). As a result, an upgradation in its interactive properties is observed that is thought to improve its binding strength.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

1] Lau, C.K.Y., Turner, L., Jespersen, J.S., Lowe, E.D., Petersen, B., Wang, C.W., Petersen, J.E.V Lusingu,J., Theander, T.G., Lavstsen, T., Higgins, M.K. (2015) Cell Host Microbe 17: 118 DOI: 10.2210/pdb4V3E/pdb