Composite

Part:BBa_K3482004

Designed by: Thierry Marti   Group: iGEM20_UNILausanne   (2020-10-18)
Revision as of 15:17, 20 October 2020 by Jeremy95 (Talk | contribs) (→‎Characterization)


IPTG-inducible miniColicin toxin

This part allows for IPTG inducible expression of the DNAase miniColicin in E. coli. The degradation of DNA , which leads to cell death, can therefore be induced by IPTG.


Biology

Colicin E2 is an endonuclease that cut on both single and double-stranded DNA. It has no specific cutting site. Colicin toxin are toxins that can be produced by E.coli and closely related bacteria.

Usage

We used that part to test if the expression of the toxin work in our E.coli nissle 1917 strain and if it was sufficient do provoc cell death

Characterization

File:BBa K3482004 kill-switch plate.File:File.png
Functional killswitch assay with IPTG and aTc gradients on agar plate

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 124
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None