Protein_Domain

Part:BBa_K3582004:Design

Designed by: Saillesh Aravindhan C   Group: iGEM20_IISER-Pune-India   (2020-10-20)
Revision as of 15:14, 20 October 2020 by Sailleshac (Talk | contribs) (→‎Design Notes)


The DBLb domain of PF11_0521 PfEMP1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1407
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1113
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • 1] RFC10 criteria satisfied
  • 2] 6x-His-tag gene added for purification
  • 3] Codon optimized with E-coli K-12 strain

Source

Protein sequence was obtained from PDB and reverse translated to give this biobrick

References