Composite

Part:BBa_K3328025:Experience

Designed by: Qi Wu   Group: iGEM20_OUC-China   (2020-10-04)
Revision as of 15:00, 20 October 2020 by Felix Felicis (Talk | contribs) (→‎Applications of BBa_K3328025)


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Applications of BBa_K3328025

In the NIMPLY gate, a deactivating RNA (INPUT A) uses direct hybridization or strand displacement to abolish trigger RNA (input B) activity. So when only the correct trigger RNA is expressed, the switch can be turned on.

The left figure shows that when INPUT A=0, INPUT B=1, the fluorescence of GFP is high. And the fluorescence intensity of GFP was low in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/ml) is added, INPUT B=1 means that HSL (0.1 mg/ml) is added.

User Reviews

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