Protein_Domain

Part:BBa_K3582000:Design

Designed by: Saillesh Aravindhan C   Group: iGEM20_IISER-Pune-India   (2020-10-20)
Revision as of 14:34, 20 October 2020 by Sailleshac (Talk | contribs) (→‎Design Notes)


ICAM-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1] Strep tag was added at the end of the biobrick for ease of purification 2] The sequence was codon optimised with the E.coli K-12 strain 3] RFC10 criteria satisfied

Source

The protein sequence was taken from PDB and was reverse translated to give this biobrick

References