Coding

Part:BBa_K3402028

Designed by: Yibo Shi   Group: iGEM20_Jiangnan_China   (2020-10-20)
Revision as of 12:16, 20 October 2020 by ZZ 0991 (Talk | contribs)


HDV

HDV is linked at the 5' end of sgGFP(BBa_K3402024).

Usage and Biology

In the device that we want to express Cas9 gene, this gene will help in the formation of mature sgRNA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 33
    Illegal NgoMIV site found at 62
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None