Coding

Part:BBa_K3402028

Designed by: Yibo Shi   Group: iGEM20_Jiangnan_China   (2020-10-20)
Revision as of 07:19, 20 October 2020 by ZZ 0991 (Talk | contribs)


HDV

This part consists of three short genes. RS and HH. are linked at the 3’ end of sgGFP(BBa_K3402024), HDV is linked at the 5’ end of sgGFP.

Usage and Biology

In the device that we want to express Cas9 protein, these three parts of genes will help in the formation of mature sgRNA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 33
    Illegal NgoMIV site found at 62
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None