Plasmid

Part:BBa_K3394009:Design

Designed by: Talia Shaler   Group: iGEM20_BGU-Israel   (2020-10-17)
Revision as of 05:58, 20 October 2020 by Talia sh (Talk | contribs) (→‎Source)

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ICE (Integrative and Conjugative Element)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 13962
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2017
    Illegal BsaI.rc site found at 13388


Design Notes

Most ICE consist a large plasmid that may "overload" our bacteria. Therfore tried to find a "mini" ICE system.


Source

Enterococcus faecalis DS16

Artificial sequence - Synthetic construct

References

Johnson, C. M., & Grossman, A. D. (2015). Integrative and conjugative elements (ICEs): what they do and how they work. Annual review of genetics, 49, 577-601.

https://db-mml.sjtu.edu.cn/ICEberg2/feature_page.php?ice_id=45

Auchtung, J. M., Aleksanyan, N., Bulku, A., & Berkmen, M. B. (2016). Biology of ICEBs1, an integrative and conjugative element in Bacillus subtilis. Plasmid, 86, 14-25.