Coding

Part:BBa_K3394003:Design

Designed by: Talia Shaler   Group: iGEM20_BGU-Israel   (2020-10-11)
Revision as of 05:41, 20 October 2020 by Talia sh (Talk | contribs) (→‎References)


Glycine-serine (GS) linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We decided to include GS linker in our design to keep the catalytic domain of endo5a separate from the cellulose-binding domain (CBD).


Source

Artificial sequence - synthetic construct

References

Lu, P., & Feng, M. G. (2008). Bifunctional enhancement of a β-glucanase-xylanase fusion enzyme by optimization of peptide linkers. Applied microbiology and biotechnology, 79(4), 579-587.