Protein_Domain

Part:BBa_K3506008:Design

Designed by: Ran Yan   Group: iGEM20_BNU-China   (2020-10-19)
Revision as of 02:33, 20 October 2020 by Yanran (Talk | contribs) (→‎References)


Linker peptide KKKKS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Synthesized de novo by GENEWIZ

Its codon has been optimized based on Codon Usage frequency in highly expressed genes of yeast



Source

We found the amino acid Sequence from Hendrickson, C. , Iii, M. A. M. , Morabito, L. , & Holloway, S. L. . (2001). The ken box regulates clb2 proteolysis in g1 and at the metaphase-to-anaphase transition. , 11(22), 1781-1787.

References

[1]Hendrickson, C. , Iii, M. A. M. , Morabito, L. , & Holloway, S. L. . (2001). The ken box regulates clb2 proteolysis in g1 and at the metaphase-to-anaphase transition. , 11(22), 1781-1787.

[2]Sharp, P. M., Tuohy, T. M., & Mosurski, K. R. (1986). Codon usage in yeast: cluster analysis clearly differentiates highly and lowly expressed genes. Nucleic acids research, 14(13), 5125–5143. https://doi.org/10.1093/nar/14.13.5125