Part:BBa_K3506008

Linker peptide KKKKS

This part can be used as a protein linker in yeast. Its nucleotide sequence is aagaagaagaagtcc. Its codon has been optimized based on Codon Usage frequency in highly expressed genes of yeast(Figure 1). We designed this part to fuse the first 124 amino acids of Clb2 and Cas9.

Usage

This part can be used to fuse two proteins in yeast. If you want to fuse two proteins, you can add this part between your sequences by designing primers containing the sequence of this part.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1]Hendrickson, C. , Iii, M. A. M. , Morabito, L. , & Holloway, S. L. . (2001). The ken box regulates clb2 proteolysis in g1 and at the metaphase-to-anaphase transition. , 11(22), 1781-1787.

[2]Sharp, P. M., Tuohy, T. M., & Mosurski, K. R. (1986). Codon usage in yeast: cluster analysis clearly differentiates highly and lowly expressed genes. Nucleic acids research, 14(13), 5125–5143. https://doi.org/10.1093/nar/14.13.5125