Coding

Part:BBa_K3332011

Designed by: Xiaoyu Wang   Group: iGEM20_XMU-China   (2020-10-19)
Revision as of 23:50, 19 October 2020 by Registry (Talk | contribs)

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GRHPR-his-tag

The enzyme efficiently catalyzes the reaction of reducing glyoxalic acid and consuming NADPH. His-tag was added to purify the protein. We use K880005 to construct the expression system and to express and to purify the protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 547
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/basic_part/nominee
Parameters
None