Composite

Part:BBa_K3431046

Designed by: Jian-An Pan, Cheng-Yang Ma, Yi-Ching Chen, Shen-Lin Chen, Huan-Jui Chang   Group: iGEM20_CSMU_Taiwan   (2020-10-17)
Revision as of 17:11, 19 October 2020 by IrisRChen (Talk | contribs)


or146_B_ToeholdSwitch-Regulated Invertase

Toehold Switch with Invertase as Expression Protein (or146_B) is an RNA-based device that is to apply as a biosensor for miRNA. This biosensor is designed to detect and reflect the amount of miRNA-146 through the expression of Beta-Fructosidase (Thermotoga Maritima MSB8) (BBa_K3431000), which can convert sucrose into glucose in a time-saving process and its result can be easily represented in the readout of glucose meter. The mechanism for detection relied on the following part - Toehold Switch for miRNA-146 (or146_B) (BBa_K3431038) - whose restriction on the expression of invertase can be liberated upon binding with miRNA-146. Furthermore, we include T7 promoter (BBa_I719005) and terminator (BBa_K731721) to insure that our device can transcribe and translate in the environment of PURExpress in vitro protein synthesis kit.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 40
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 40
    Illegal NheI site found at 1428
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 40
    Illegal BglII site found at 1199
    Illegal BamHI site found at 1329
    Illegal XhoI site found at 1400
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 40
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 40
    Illegal AgeI site found at 1000
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None