Tag

Part:BBa_K3351009

Designed by: Yu Zhang   Group: iGEM20_NWU-CHINA-A   (2020-09-08)
Revision as of 13:18, 19 October 2020 by ZZ-NWU (Talk | contribs)


His tag.

His tags have gained great popularity over the last decade as a purification tool for recombinant proteins. Cloning vectors generally introduce six consecutive histidines and an optional protease-cleavage site or linker to the N- or C-terminus of the protein of interest. These His tags facilitate selective binding of the expressed protein to a nickel-affinity column. The tag may then optionally be removed by a protease, requiring another purification step.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None