Regulatory

Part:BBa_K3506007

Designed by: Ran Yan   Group: iGEM20_BNU-China   (2020-10-19)
Revision as of 12:47, 19 October 2020 by Registry (Talk | contribs)

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CLB2 promoter

This part is the CLB2 promoter which is -829 to -365 nt relative to CLB2 ATG on the genome of Saccharomyces cerevisiae BY4741. CLB2 promoter is the promoter of the G2/mitotic-specific cyclin-2(CLB2) which accumulates steadily during G2 and is abruptly destroyed at mitosis. CLB2 interacts with the CDC2 protein kinase to form maturation-promoting factor(MPF) and is essential for the control of the cell cycle at the G2/M (mitosis) transition.Previous studies have revealed that CLB2 promoter can regulate the transcription of its downstream gene periodically. Under the control of CLB2 promoter, the downstream gene starts to transcript in the S phase, reaches a peak at the G2/M transition, and rapidly degrades at the end of the M phase.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 229
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 203
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None