Regulatory
Part:BBa_K3629001:Design
Designed by: Sravya Kakumanu Group: iGEM20_Calgary (2020-10-09)
Yarrowia lipolytica TEF1 promoter with an intron
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The wild-type sequence of this promoter contains BsaI, PstI, and SpeI restriction sites in the promoter section before the intron sequence. Therefore, single-nucleotides changes that match the ones in the functional BBa_K2983050 promoter were made to remove these sites.
Source
From the genomic sequence of wild-type Y. lipolytica strain CLIB122.