Coding

Part:BBa_K3629008

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-10-12)
Revision as of 02:45, 19 October 2020 by Sravyakakumanu (Talk | contribs) (→‎Design)


Modified Trichoderma reesei EGI with 6X His tag

Temperature and pH optimized endoglucanase I coding sequence from Trichoderma reesei with 6x His tag.

Usage and Biology

Design

The native signal peptide from T. reesei was removed so it would not interfere with fused secretion tags native to Y. lipolytica

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 523
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[edit]
Categories
//awards/part_collection
//chassis/eukaryote/yeast
Parameters
None