Coding

Part:BBa_K3629007

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-10-12)
Revision as of 02:44, 19 October 2020 by Sravyakakumanu (Talk | contribs) (→‎Design)


Trichoderma reesei CBHII with FLAG tag

Cellobiohydrolase II coding sequence from Trichoderma reesei with FLAG tag.

Usage and Biology

Design

The native signal peptide from T. reesei was removed so it would not interfere with fused secretion tags native to Y. lipolytica

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 341
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 601
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[edit]
Categories
Parameters
None