DNA
Part:BBa_K3577000
Designed by: Ruyi Shi Group: iGEM20_Worldshaper-Shanghai (2020-07-27)
Revision as of 02:38, 19 October 2020 by SylviaSong (Talk | contribs)
T7-toehold switch-mCherry
The function of this part is to produce toehold switch in vitro. It consists of T7 promoter, toehold switch (BBa_K3577001), mCherry and T7 terminator. Toehold switch is a special RNA hairpin structure, which contains trigger strand, ribosome binding site, translation start codon and a report gene. Our toehold switch sequence (BBa_K3577001) is based on an article called Toehold Switches: De-Novo-Designed Regulators of Gene Expression (Green A, et al., 2014) and the mCherry is added to the downstream of the RNA hairpin structure.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |