Protein_Domain

Part:BBa_K3487013

Designed by: RuiXin Lin   Group: iGEM20_SZPT-CHINA   (2020-10-18)
Revision as of 16:15, 18 October 2020 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Tachyplesin I multimers with GST-tag

The GST-tag has the size of 220 amino acids (roughly 26 KDa). It is fused to the N-terminus of the Tachyplesin I multimers BBa_K3487001 and includes a PreScission Protease domain for cleavage of the GST tag during protein purification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 673
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


[edit]
Categories
Parameters
None