Coding

Part:BBa_K3468026

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-18)
Revision as of 15:53, 18 October 2020 by Registry (Talk | contribs)

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PETase A40P,S54W,T113P,T116R,Q119D,Q127L,Q182I,S193R,S214F,K252L,R260F,T266P

The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed A40P,S54W,T113P,T116R,Q119D,Q127L,Q182I,S193R,S214F,K252L,R260F,T266P position which can be more stable in higher temperature compared with the wild type.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 240
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 240
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 240
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 240
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 240
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None