Coding

Part:BBa_K3352001

Designed by: Joyce Ting   Group: iGEM20_TAS_Taipei   (2020-10-18)
Revision as of 15:50, 18 October 2020 by Joyceting (Talk | contribs)


Φ29 DNA Polymerase with His-Tag and GS linker Sequence

Construct Design

We obtained the amino acid sequence of phi29 DNA polymerase from Bacillus phage phi29. We proceeded to optimize the DNA sequence for expression in E. coli and removed the EcoRI cutting site. We attached a 6x his-tag upstream of the phi29 DNA Polymerase for purification purposes followed by a glycine-serine linker (GS linker) to form our ORF.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 595
    Illegal SapI.rc site found at 993


[edit]
Categories
//awards/basic_part/winner
Parameters
None