Reporter

Part:BBa_K3526006

Designed by: Kong Yangyang   Group: iGEM20_Shanghai_city   (2020-10-18)
Revision as of 13:38, 18 October 2020 by Registry (Talk | contribs)

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tac pro-atzR-T4 ter-PatzD-EGFP-T7 ter

The transcription factor atzR can sense the presence of cyanuric acid, and then regulate the promoter PatzD to start expressing EGFP protein, and finally emit green fluorescence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1540
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1026
    Illegal AgeI site found at 864
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None