![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K3487001
Designed by: Jianhua Zhou Group: iGEM20_SZPT-CHINA (2020-10-08)
Revision as of 12:59, 18 October 2020 by LiuWanTing (Talk | contribs)
TP-ⅠM,A polymer of Tachyplesin Ⅰ
Description
A long-chain protein formed by polymerizing four identical short TP-I monomer peptides.
Usage and Biology
After the tp1 polymer is expressed, it is inactive. It will be expressed as a long-chain protein by the engineered bacteria and taken to the extracellular environment by the signal peptide and then hydrolyzed into four active short peptides.
![](/wiki/images/b/bd/T--SZPT-CHINA--TP-1M.png)
By externally synthesizing an expression vector with Tachyplesin I multimers, transferring the vector into BL21 for antibiotic screening to obtain a single clone, culture it in liquid medium to OD600 and add IPTG to induce 12 hours to obtain recombinant protein, and collect After ultrafiltration and concentration, purified Tachyplesin I polymer was obtained. SDS-PAGE electrophoresis identification showed that the target protein had been obtained.
Characterization
By externally synthesizing an expression vector with Tachyplesin I multimers, transferring the vector into BL21 for antibiotic screening to obtain a single clone, culture it in liquid medium to OD600 and add IPTG to induce 12 hours to obtain recombinant protein, and collect After ultrafiltration and concentration, purified Tachyplesin I polymer was obtained. SDS-PAGE electrophoresis identification showed that the target protein had been obtained.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |