Regulatory

Part:BBa_K3487007

Designed by: Jianhua Zhou   Group: iGEM20_SZPT-CHINA   (2020-10-09)
Revision as of 11:59, 18 October 2020 by LiuWanTing (Talk | contribs)


Lysine riboswitch

Description

Usually the suicide switch that prevents organisms from escaping will be set to auxotrophic type, which requires gene resection technology, and whether it is accurate is a big problem that needs to be faced. The riboswitch does not have these problems. The gene sequence is directly introduced into the required vector, and the switch directly forms a multiple stem-loop structure on the mRNA to stop translation.

T--SZPT-CHINA--RS.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 12
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 284
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 256

References

【2】Garst AD, Héroux A, Rambo RP, Batey RT. Crystal structure of the lysine riboswitch regulatory mRNA element. J Biol Chem. 2008;283(33):22347-22351.

[edit]
Categories
//rna/riboswitch
Parameters
None