Part:BBa_K3633014

A composite part to express Tyr1 induced by IPTG

In this part, the gene of Tyr1 is added to promoter pTac-RBS.

Tyr gene provides instructions for making enzyme tyrosinase which is located in melanocytes in our skin. Melanocytes are responsible for producing melanin. Melanin is responsible for giving our skin and hair color. It includes eumelanin and pheomelanin. Eumelanin usually shows darker color than pheomelanin. As shown below, tyrosinase is responsible for the first step in melanin production.

Tyrosine is one of the 20 standard amino acids present in the body and used by cells to synthesize proteins. This is a non-essential amino acid, meaning that when it is not only ingested from the diet, it is also synthesized by the body. Tyrosine is found in casein, a protein in milk and other milk-based products (like cheese). Tyrosine is also present in non-dairy foods that are aged, including some types of meats and red wine.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 376
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]