Part:BBa_K3431023

zr31_ToeholdSwitch-Regulated Invertase

Toehold Switch with Invertase as Expression Protein (zr31) is an RNA-based device that is to apply as a biosensor for miRNA. This biosensor is designed to detect and reflect the amount of miRNA-31 through the expression of Beta-Fructosidase (Thermotoga Maritima MSB8) (BBa_K3431000), which can convert sucrose into glucose in a time-saving process and its result can be easily represented in the readout of glucose meter. The mechanism for detection relied on the following part - Toehold Switch for miRNA-31 (zr31) (BBa_K3431007) - whose restriction on the expression of invertase can be liberated upon binding with miRNA-31. Furthermore, we include T7 promoter (BBa_I719005) and terminator (BBa_K731721) to insure that our device can transcribe and translate in the environment of PURExpress in vitro protein synthesis kit.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1426
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1197
  Illegal BamHI site found at 1327
  Illegal XhoI site found at 1398
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 998
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 529