Translational_Unit

Part:BBa_K3524004

Designed by: Kong Yangyang   Group: iGEM20_Nanjing_high-school   (2020-10-18)
Revision as of 08:55, 18 October 2020 by Registry (Talk | contribs)

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Psal pro-EGFP-T7 ter

The bio-sensor (nahR and Psal) for the detection of salicylic acid we adopted is originally on the chromosome of Pseudomonas Stutzeri. With the appearance of salicylic acid, the protein translated from nahR binds with Psal promoter and initiates the expression of the enzyme that can utilize the acid as one of the energy sources for Pseudomonas Stutzeri. In our design, we replaced the essential gene with the GFP gene to test our framework. With GFP, the situation is that the edited bacteria can be fluorescent with the existence of salicylic acid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None