![](https://parts.igem.org/images/partbypart/icon_device.png)
Device
Part:BBa_K3629018:Design
Designed by: Sravya Kakumanu Group: iGEM20_Calgary (2020-10-18)
N. patriciarum BGS expression construct with 6X His tag
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3112
Illegal SpeI site found at 253
Illegal PstI site found at 218 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3112
Illegal NheI site found at 44
Illegal SpeI site found at 253
Illegal PstI site found at 218 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3112
Illegal BglII site found at 660
Illegal BglII site found at 2641
Illegal BamHI site found at 973 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3112
Illegal SpeI site found at 253
Illegal PstI site found at 218 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3112
Illegal SpeI site found at 253
Illegal PstI site found at 218
Illegal NgoMIV site found at 1484 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
x
Source
The coding sequence of the BGS is from Neocallimastic patriciarum, however it has been codon-optimized to have optimal activity in Yarrowia lipolytica. The promoter and terminator sequences are from wild-type Y. lipolytica.