Device
Part:BBa_K3629016
Designed by: Sravya Kakumanu Group: iGEM20_Calgary (2020-10-18)
Revision as of 07:24, 18 October 2020 by Sravyakakumanu (Talk | contribs)
Modified T. reesei EGI expression construct with gibson homology sequences and 6X His tag
Usage and Biology
Design
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2385
Illegal EcoRI site found at 2204 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal EcoRI site found at 2204
Illegal NheI site found at 81
Illegal SpeI site found at 2386
Illegal PstI site found at 2400
Illegal NotI site found at 7
Illegal NotI site found at 2393 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal EcoRI site found at 2204
Illegal BamHI site found at 2334
Illegal XhoI site found at 132 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2386
Illegal EcoRI site found at 2204 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal EcoRI site found at 2204
Illegal XbaI site found at 16
Illegal SpeI site found at 2386
Illegal PstI site found at 2400
Illegal NgoMIV site found at 1326 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |